Fig. 3: On-chip analysis of MCF-7 cells exposed to FSS exhibit enhanced Ki67 protein levels as a marker of proliferation. | Microsystems & Nanoengineering

Fig. 3: On-chip analysis of MCF-7 cells exposed to FSS exhibit enhanced Ki67 protein levels as a marker of proliferation.

From: A modular microfluidic platform to study how fluid shear stress alters estrogen receptor phenotype in ER+ breast cancer cells

Fig. 3

a Representative immunostaining images of single MCF-7 cells exposed to shear at a magnitude of 10 dyn/cm2 for Ki67 (proliferation, green) and DAPI (nuclear, blue). b Representative immunostaining images of single non-sheared MCF-7 cells for Ki67 (proliferation, green) and DAPI (nuclear, blue). c Quantification and distribution of normalized fluorescence in single cells coupled with one-way ANOVA which shows statistically significant changes between the sheared population and non-sheared population (*** indicates statistically significant p < 0.001, ns indicates statistically non-significant p > 0.05). The data is representative of n = 3 technical replicates

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