Fig. 4: Exposure to FSS results in AKT phosphorylation in MCF-7 cells in both on-chip and off-chip experiments. | Microsystems & Nanoengineering

Fig. 4: Exposure to FSS results in AKT phosphorylation in MCF-7 cells in both on-chip and off-chip experiments.

From: A modular microfluidic platform to study how fluid shear stress alters estrogen receptor phenotype in ER+ breast cancer cells

Fig. 4

a Immunostaining images of AKT phosphorylation (p-AKT, green) and DAPI (nuclear, blue) in non-sheared and sheared cells at 10 dyn/cm2. b Quantification and distribution of normalized fluorescence in single cells coupled with one-way ANOVA which shows statistically significant changes between the sheared population and non-sheared population. c Western blot assay for p-AKT (Ser473). d Graphical representation of the normalized band density. The amount of phosphoprotein was normalized to Rho GDIα, and amount of phosphoprotein from the non-Sheared population was standardized at 1. Data is representative of n = 2 biological replicates, mean ± SEM. (*** indicates statistically significant p < 0.001, * indicates statistically significant p < 0.05, ns indicates statistically non-significant p > 0.05)

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