Fig. 3: SEM images of the Phycomyces blakesleeanus cell wall after the femtosecond laser nanosurgery. | Microsystems & Nanoengineering

Fig. 3: SEM images of the Phycomyces blakesleeanus cell wall after the femtosecond laser nanosurgery.

From: In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi

Fig. 3: SEM images of the Phycomyces blakesleeanus cell wall after the femtosecond laser nanosurgery.

a TPEF image of the fs laser-generated incision in the hyphal cell wall. The average laser power in the sample plane was 1.0 mW for imaging (dwell time 2.5 µs) and 7.5 mW for surgery (dwell time 1 s). Scale bar: 5 µm. b SEM image of the same laser-generated incision shown in a. Scale bar: 2 µm. c TPEF image of the fs laser-made incision. The average laser power in the sample plane was 1.0 mW for imaging (dwell time 2.5 µs) and 7.6 mW for surgery (dwell time 1 s). Scale bar: 5 µm. All TPEF images were acquired with a Zeiss 40× 1.3 oil objective. The color intensity bars for the TPEF signal are as follows: violet/blue, lowest TPEF signal; and dark red, highest TPEF signal. The color intensity bar is linear and covers the entire range of the data. d SEM image of the entire laser-generated incision, same as that shown in c. Scale bar: 2 µm. e Hyphal cell wall thickness (d) of the incision shown in d. Scale bar: 0.5 µm. f Released protoplast through a laser incision made with 6.7 mW average power at 730 nm. Scale bar: 1 µm

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