Fig. 7: Schematic drawing of the NLSM setup for nanosurgery and cell wall imaging. | Microsystems & Nanoengineering

Fig. 7: Schematic drawing of the NLSM setup for nanosurgery and cell wall imaging.

From: In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi

Fig. 7: Schematic drawing of the NLSM setup for nanosurgery and cell wall imaging.

Ti:Sa fs laser for cell surgery and TPEF imaging, VNDF motorized variable neutral density filter, Sh shutter, GSM galvanometer-scanning mirrors, L1 and L2 - beam expander, MDM main dichroic mirror (cutoff 700 nm), Obj. microscopic objective 40 × 1.0 physiological, Sam. sample, Con. aspheric condenser lens, TL tube lens, BS/M beam splitter or mirror toggle, Cam. camera, F VIS filter 400–700 nm for CFW fluorescence, L3, L4 focusing lens, TPEF PMT photomultiplier tube for TPEF signal, PD photodiode, AD/DA acquisition card

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