Fig. 1: Construction and characterization of the THE signal amplification system. | Microsystems & Nanoengineering

Fig. 1: Construction and characterization of the THE signal amplification system.

From: Ultra-sensitive fluorescence-activated droplet single-cell sorting based on Tetramer-HCR-EvaGreen amplification

Fig. 1

a The schematic of the HCR isothermal amplification. A dendritic DNA nanostructure was constructed by O-trigger strand with Substrate-A, Substrate-B, Assistant-A and Assistant-B. The Apt-TDNs-dendrimers would be formed by initiating HCR on three vertexes of Apt-TDNs. The THE signal amplification system would be constructed by introducing EvaGreen into the Apt-TNDs-dendrimers. b PAGE characterization of the Apt-TDNs-dendrimers. Lane1, 25–500 bp marker; Lane2-5, Apt-TDNs-dendrimers formed when Apt-TDNs solution with different concentration (0.05, 0.1, 0.25, 0.5 μM) were mixed with H-solution; lane6, H solution; lane7, 1 uM Apt-TDNs solution; lane8-9, two HCR substrate, Substrate-A and Substrate-B solutions. c The AFM characterization of the Apt-TDNs and Apt-TDNs-dendrimers. Left: Apt-TDNs, right: Apt-TDNs-dendrimers. The scale bar is 1000 nm. d Bar chart of fluorescence intensity quantification by microplate reader for H solution, E solution, HCR and HCR plus EvaGreen. The error bars represented the standard deviation of three measurements. All measurements were performed in triplicate, and error bars represented the ± SD. ****P < 0.0001, Student’s t-test

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