Fig. 3: Construction of the THE-FADS. | Microsystems & Nanoengineering

Fig. 3: Construction of the THE-FADS.

From: Ultra-sensitive fluorescence-activated droplet single-cell sorting based on Tetramer-HCR-EvaGreen amplification

Fig. 3

a Design of the microfluidic chip used for the THE-FADS. The three inlets on the bottom shows the region of droplet making (a, blue), droplet sorting (b, yellow) and droplet collection (c, green). The scale bar is 200 μm. b Distribution of peak signal intensity across various group. From top to bottom, the rows a, b, c and d represent the control group, antibody group, HCR group, and HCR-Eva group, respectively. c Confocal fluorescence images of the obtained droplets under unsorting and sorting mode, respectively. When no sorting voltage is applied, a few droplets have MCF-7 cells (upper). When sorting voltage is applied, most of the droplets have MCF-7 cells (lower). The scale bar is 200 μm

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