Fig. 4: MCF-7 cell sorting by the THE-FADS in mimic serum sample and downstream analysis. | Microsystems & Nanoengineering

Fig. 4: MCF-7 cell sorting by the THE-FADS in mimic serum sample and downstream analysis.

From: Ultra-sensitive fluorescence-activated droplet single-cell sorting based on Tetramer-HCR-EvaGreen amplification

Fig. 4

a The schematic diagram of the experiment design. b Confocal fluorescence images of the sorted droplets containing MCF-7 cells from mimic serum sample. From 1 to 4: green fluorescence channel image, blue fluorescence channel image, bright field image and merge image. The scale bar is 200 μm. c Confocal images of the recultured MCF-7 cells obtained from THE-FADS in a mimic serum sample after 3 days culture. The scale bar is 50 μm. d Bar chart of EGFR mRNA and CK mRNA expression profile for recultured THE-FADS obtained MCF-7 cells (left) and control MCF-7 cells (right). All measurements were performed in triplicate, and error bars represented the ± SD

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