Fig. 1: Structure, fabrication, and detection process of the VFPT-based analytical platform. | Microsystems & Nanoengineering

Fig. 1: Structure, fabrication, and detection process of the VFPT-based analytical platform.

From: Vertical-flow tearable paper-tape rolls for scalable multiplexed point-of-care nucleic acid testing

Fig. 1: Structure, fabrication, and detection process of the VFPT-based analytical platform.

a VFPT-tape roll: The VFPT is prepared as a roll, similar to tape. The colored areas are made of tape, and the white circular areas are cellulose filter paper. A single VFPT, with multiple functional pads (3 cm per side), is torn off and folded for use. b Roll-to-Roll Fabrication: The VFPT is mass-fabricated using a roll-to-roll process, with the fabricated VFPT-roll shown in the image. c VFPT-Based Platform Workflow: The VFPT is used for sample preparation and nucleic acid extraction through four steps via vertical flow. The folded multiplexed detection pad is then flattened and placed on the homemade portable analysis platform (PAP) for isothermal amplification. Results are quantitatively read and analyzed through a smartphone app

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