Fig. 4: Spheroid trapping and simulation based on the mechanical properties of different lineages.

a Simulated selective trapping of adipogenic spheroids based on assumed density variations. b Trapping forces as a function of spheroid diameter. c Two-dimensional acoustic potential wells as a function of assumed spheroid density. d Three-dimensional acoustic potential wells as a function of assumed spheroid density. Two-dimensional manipulation of trapped adipogenic spheroids in the upward e and rightward f directions. g Deformation of adipogenic spheroids under increasing acoustic pressure and continuous trapping. h Progressive increase in deformability of adipogenic spheroids over time and under increased acoustic pressures. All scale bars indicate 200 μm