Fig. 3: Vulvar in situ (dVIN) and invasive squamous cell carcinoma (VSCC) showing p53 IHC patterns inconsistent with TP53 mutation status.

a This VSCC had three different TP53 missense mutations but showed scattered p53 staining (Case 23). b VSCC had an absent pattern staining in the presence of positive staining in the background inflammatory cells but no TP53 mutation was detected (Case 57). VSCC shows predominantly absent staining (c) in the 90% of the tumor and cytoplasmic staining (d) in 10%. This tumor had a splice site mutation (Case 58). e, f Two dVIN lesions had TP53 missense mutations but exhibit scattered p53 staining (Cases 22 and 23).