Fig. 3: Effects of overexpression of FOXG1 WT and variants on neuronal migration by IUE.
From: Functional defects in FOXG1 variants predict the severity of brain anomalies in FOXG1 syndrome
A Neuronal distribution of brains electroporated with Foxg1 and the pathogenic variants. Mouse brains were electroporated with GFP along with Foxg1 WT or its variants at E13.75. Neuronal cell distribution was examined 3 days after IUE in brain slices stained with the layer marker TBR1 (red) and DAPI (blue). While more than half of the cells electroporated with the empty vector migrated to the CP, FOXG1 overexpression altered neuronal migration, causing most cells to accumulate in the VZ and IZ. Cells electroporated with pathogenic Foxg1 variants displayed varying degrees of migration alteration. Bar = 100 μm. B The bar graph shows cell distributions in the VZ, IZ, and CP after IUE. C The bar graph shows cell distributions in the CP after IUE. Dashed horizontal lines indicate 10 and 25% of cells distributed in the CP. Error bars: S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001. ****p < 0.0001. Two-way ANOVA, post-hoc: Dunnett’s multiple comparisons test (n = 3 independent IUE experiments).
