Fig. 2: RNA-seq analysis after NMDAR1-Ab exposure in hFOs.
From: The anti-NMDAR1 antibodies and IL-17 signaling pathway shape NMDAR encephalitis
a Heatmap and volcano plots for RNA-seq data of NMDAR1-Ab exposure and control hFOs. Outlier samples were excluded based on the hierarchical cluster analysis. (b, c) GO and KEGG pathway analysis for upregulated (b) and downregulated (c) DEGs in NMDAR1-Ab-exposed hFOs, respectively. GO terms are shown with red bars, and KEGG pathways are shown with blue bars. d PPI network analysis for the downregulated DEGs in NMDAR1-Ab-exposed hFOs. The top 10 hub nodes with the most connections are highlighted in red. (e, f) RT-qPCR analysis for GRIN1 and GRIA2 (The hub nodes in the PPI network) in hFOs after exposure to monoclonal NMDAR1-Ab (e) or IgG purified from CSF of NMDARE patients (f). N ≥ 3 technical replicates per group. Data are shown as Mean ± SD. Two-tailed t-test, *P < 0.05, ***P < 0.001.
