Fig. 4: Analysis of spontaneous behavior in automated home-cage environment (PhenoTyper).
From: Phenotypic diversity is caused by non-linear genetic interactions between two SNAREopathy genes
A Statistical analysis of 90 parameters of spontaneous behavior separated into 6 categories: kinematics, activity, sheltering, habituation, dark-light index and phase transition. Three annuli show statistical analysis for double mutants, Stxbp1 single- and Snap25 single- mutants compared to their control groups, respectively from periphery to the center. The full list of parameters and statistical analysis can be found in Supplemental Tables 2–4. B-C Kinematic parameters: short movement number during the light and long movement number during the light were significantly decreased in Stxbp1 single- and double- mutants compared to control group. D Activity parameter: On shelter zone duration during the dark was significantly decreased in Stxbp1 single- and double- mutants compared to control groups. E Sheltering parameter: Long shelter visit number during light was significantly decreased in double mutants compared to Snap25 single mutants and controls. F Habituation: On shelter zone habituation ratio was significantly decreased in Stxbp1 single- and double- mutants compared to control group. Panels B - F represent mean value ± SEM.
