Fig. 6: Assessment of synaptic transmission phenotype in the autaptic hippocampal cultures in two separate experiments.

A Typical evoked responses (EPSC) in control-, Stxbp1 single-, Snap25- single and double- mutant glutamatergic neurons in 2 mM / 2 mM Ca²⁺/Mg²⁺ concentrations. B Normalized EPSC per week. C Representative traces of 500 mM sucrose responses. D Normalized readily releasable pool (RRP) of glutamatergic neurons calculated from hypertonic sucrose responses. E Typical inhibitory evoked response (IPSC) in control, Stxbp1 single-, Snap25 single- and double- mutants GABA-ergic neurons in 2 mM / 2 mM Ca²⁺/Mg²⁺ concentrations. F Normalized IPSC per week. G Representative traces of 500 mM sucrose responses in GABA-ergic neurons. H Normalized RRP in GABA-ergic neurons. I Normalized EPSC per week in control-, Stxbp1 single -, Snap25 single- and double- mutant glutamatergic neurons in 2 mM / 4 mM Ca²⁺/Mg²⁺ concentrations. J Normalized paired pulse ratio (PPR) per week per group for paired-pulse intervals of 20 ms, 50 ms and 100 ms. K Cumulative charge during train-stimulation (40 Hz, 100 pulses) with back-extrapolation line of last 20 pulses used for estimation of RRP. L Normalized synaptic rundown of EPSC during first ten pulses of 40 Hz train-stimulation.