Fig. 2: Mefenamic acid (MFA) partially improved the motor behavior deterioration in WT and Sh3^+/− mice.

A Schematic drawing for experiment design. Anti-inflammatory treatment with intraperitoneal MFA injection was given to mice once every day for 7 days after LPS injection. Rotarod tests were performed 24 h after the last does of MFA injection. B-C. MFA partially improved the rotarod test performance. After 1-week treatment of MFA, steady speed rotarod (16 rpm, B) and accelerated speed rotarod (4–40 rpm, C) tests were performed and latency to fall was compared. (Two-way ANOVA, B. 16 rpm, LPS/MFA effect F _{(1, 37)} = 23.66, p < 0.0001, genotype effect F _(1,46) = 1.859, p = 0.1794, LPS/MFA X genotype interaction F _{(1, 37)} = 0.8697, p = 0.3571. C. 4–40 rpm, LPS/MFA effect F _{(1, 85)} = 1.721, p = 0.1932, genotype effect F _(1,85) = 4.822, p = 0.0308, LPS/MFA X genotype interaction F _{(1, 85)} = 0.8220, p = 0.3672). Compared to vehicle group, MFA-treated Sh3^+/− and WT mice showed increased latency to fall in 16 rpm rotarod test (Sh3^+/−, p = 0.0091; WT, p = 0.0002). D-E. MFA treatment was more effective in female Sh3^+/− mice than male ones. Two-way ANOVA was performed to compare sex difference in MFA treatment (D. 16 rpm, LPS/MFA effect F _{(1, 43)} = 4.626, p = 0.0372, sex effect F _(1,43) = 9.227, p = 0.004, LPS/MFA X sex interaction F _{(1, 43)} = 1.858, p = 0.1799. E. 4–40 rpm, LPS/MFA effect F _{(1, 16)} = 2.065, p = 0.17, sex effect F _(1,29) = 2.369, p = 0.1346, LPS/MFA X sex interaction F _{(1, 16)} = 8.046, p = 0.0119). MFA-treated female Sh3^+/− showed significantly higher latency to fall compared to vehicle-treated female Sh3^+/− (16 rpm, p = 0.0229; 40 rpm, p = 0.0106) and MFA-treated male Sh3^+/− (16 rpm, p = 0.0009; 40 rpm, p = 0.0011). ^* p < 0.05, ^** p < 0.005, ^*** p < 0.0005, ^**** p < 0.0001.