Fig. 3: Bulk RNA sequencing revealed distinct molecular mechanism underlying LPS effect on Sh3+/− mice. | Molecular Psychiatry

Fig. 3: Bulk RNA sequencing revealed distinct molecular mechanism underlying LPS effect on Sh3+/− mice.

From: Inflammation increases the penetrance of behavioral impairment in Shank3 haploinsufficiency mice — can it explain the behavioral regression in Autism?

Fig. 3: Bulk RNA sequencing revealed distinct molecular mechanism underlying LPS effect on Sh3+/− mice.

A–D. The volcano plots show differentially expressed genes (DEGs) distribution. DEGs were identified in four comparisons: Sh3+/−+LPS vs Sh3+/−+PBS (A), WT + LPS vs. WT + PBS (B), Sh3+/−+LPS vs. WT + LPS (C), Sh3+/−+PBS vs. WT + PBS (D). Upregulated genes are indicated in red color, downregulated genes are indicated in blue color and DEGs below threshold are indicated in grey color. E-F. The venn diagrams show shared upregulated and downregulated genes among four comparisons. Shared upregulated (E) and downregulated genes (F) numbers are shown within the diagrams. Comparison of Sh3+/−+LPS vs Sh3+/−+PBS is red diagram, WT + LPS vs. WT + PBS is blue diagram, Sh3+/−+LPS vs. WT + LPS is green diagram, and Sh3+/−+PBS vs. WT + PBS is yellow diagram. G. The bubble chart shows functions of DEGs of Sh3+/−+LPS vs. WT + LPS comparison. Volcano plot of the negative log of p-value (Y-axis) vs. the z-score (X-axis). p-value was calculated using the right-tailed Fisher’s Exact Test. A negative log of p-value cutoff of 1.3 (p < 0.05) was used to identify significantly changed functional pathways. A z-score range cutoff of ±0.1 was used to identify the activation or inhibition state of a canonical pathway. Some names of pathways are annotated in the chart. Activated pathways are orange bubbles and inhibited pathways are blue bubbles. The size of the bubbles reflects the number of genes overlapped with the pathway database.

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