Fig. 4: Treatment using LNT-induced Treg cells suppresses the diabetogenicity of NOD mice. | Nutrition & Diabetes

Fig. 4: Treatment using LNT-induced Treg cells suppresses the diabetogenicity of NOD mice.

From: Lentinan confers protection against type 1 diabetes by inducing regulatory T cell in spontaneous non-obese diabetic mice

Fig. 4

Subsequent to the purification of Treg cells from euglycemic PBS (Ctrl) or LNT-treated mice (as depicted in Fig. 1A and in splenic T cells isolated 15 days after receiving the final LNT injection), Treg cells were adoptively transferred to female NOD/Ltj mice at 8 weeks (A–C) or 10 weeks (D–F) of age (intravenous; 1 × 105 Treg cells/mouse), followed by weekly monitoring of hyperglycaemia. Mice with two consecutive weeks of 250 mg/dL blood glucose levels were considered to suffer from diabetes. A and D) The frequency of diabetes-free mice over time. B and E Representative histology pancreas slices from the mice in (A) and (D). Pancreatic islets are indicated by white arrows. C and F The frequency of islets with grade 0–4 insulitis from the mice in (B) and (E) (n = 20). Summary data are summarised as the mean ± SEM. **p < 0.01.

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