Table 1 The information of used reagents.
Reagents | Company | Clone |
|---|---|---|
Purified anti-mouse CD3 (no azide and low-endotoxin) antibody | eBioscience | 145-2C11 |
Purified anti-mouse CD28 (no azide and low-endotoxin) antibody | eBioscience | 37.51 |
Fluorochrome-conjugated anti-mouse CD4 antibody | eBioscience | RM4-5 |
Fluorochrome-conjugated anti-mouse CD8α antibody | eBioscience | 53-6.7 |
Fluorochrome-conjugated anti-mouse CD45 antibody | eBioscience | 30-F11 |
Fluorochrome-conjugated anti-mouse CD25 antibody | eBioscience | PC61.5 and eBio7D4 |
Fluorochrome-conjugated anti-mouse/rat Foxp3 antibody | eBioscience | FJK-16a |
Fluorochrome-conjugated anti-mouse IL-4 antibody | eBioscience | 11B11 |
Fluorochrome-conjugated anti-mouse IL-17A antibody | BioLegend | TC11-18H10.1 |
Fluorochrome-conjugated anti-mouse IFN-γ antibody | BioLegend | XMG1.2 |
Fluorochrome-conjugated anti-mouse CD45.1 antibody | Miltenyibiotec | A20 |
Fluorochrome-conjugated anti-mouse CD45.2 antibody | Miltenyibiotec | 104-2 |
