Fig. 3: E4orf1 expression in APP/PS1 mice protects against liver lipid accumulation.

Improved glycemic control with lower endogenous insulin secretion in E4orf1 expressing APP/PS1 mice prevents lipid accumulation in the liver as seen with significantly lower de novo lipogenesis, FASn (A, B) and ACC (A, C). Protein expression was normalized to GAPDH or total protein (A) and densitometric analysis of the protein bands was plotted (B, D). Hepatic gene expression also shows significantly lower lipogenesis related gene expression (FASn, ACC and Scd1) (E), while there was no significant difference in liver fat oxidation (F) in these mice. For gene expression APP/PS1 (n = 4) and APP/PS1/E4orf1 (n = 5). Welch’s t test: *p < 0.05, **p < 0.01.