Fig. 3: VD played a role in the effect of TMAO on perirenal fat and kidney in mice with DKD. | Nutrition & Diabetes

Fig. 3: VD played a role in the effect of TMAO on perirenal fat and kidney in mice with DKD.

From: Vitamin D is involved in the effects of the intestinal flora and its related metabolite TMAO on perirenal fat and kidneys in mice with DKD

Fig. 3: VD played a role in the effect of TMAO on perirenal fat and kidney in mice with DKD.

A kidney tissue of different groups after 1,25-(OH)2D3 intervention. B Gross anatomy of perirenal adipose tissue. C Comparison of fat mass (n = 6 mice per group). D HE staining for Perirenal adipose tissue (18 images per 6 biological replicates for each group). Scale bar, 200×. E Electron microscopy for Perirenal adipose tissue in DKD and1,25-(OH)2D3 intervention group(VD) (n = 6 mice per group). Red arrow: mitochondria, blue arrow: lipid droplets, N: nucleus. Scale bar, 100 μm. (25,000×). F–I IHC-stained (with an anti-PGC1α, UCP1 and VDR antibody) (18 images per 6 biological replicates for each group) sections of PPAT and kidney. Scale bar, 50μm. (200×). J Oil stained (18 images per 6 biological replicates for each group). (400×). K Kidney and PRAT MCP-1, PGC1α and UCP-1 mRNA expression (1 technical replicate of 6 biological replicates per group).

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