Table 2 PCR primer sequences.

From: Vitamin D is involved in the effects of the intestinal flora and its related metabolite TMAO on perirenal fat and kidneys in mice with DKD

Gene

Forward primer (5′–3′)

Reverse primer (3′–5′)

PGC1α

ATGGCATGGCTTACACCACC

GAGGCCAATTTTGTCTCCACA

UCP-1

ATGGCATGGCTTACACCACC

GAGGCCAATTTTGTCTCCACA

NF-κB

AGGCTTCTGGGCCTTATGTG

TGCTTCTCTCGCCAGGAATAC

MCP-1

TCTCTTCCTCCACCACTATGCA

GGCTGAGACAGCACGTGGAT

β-actin

GGCTGTATTCCCCTCCATCG

CCAGTTGGTAACAATGCCATGT

Back to article page