Fig. 2: Blocking p-p65 alleviated ER stress and reduced the release of serum exosomal miRNAs in obese mice.
From: Palmitic acid promotes miRNA release from adipocyte exosomes by activating NF-κB/ER stress
A Gross morphology of different groups of mice. B, C Compared with those in the NCD-fed mice (n = 6), body weight and serum PA content were significantly greater in the HFD-fed mice (n = 6). Blocking p-p65 had no significant effect on body weight, but serum PA levels were reduced. D, E The protein expression level of p-p65 and the mRNA expression level of inflammatory factors were significantly increased in the abdominal adipose tissue of mice in the HFD group. Blocking p-p65 reversed the above phenotypes in HFD-fed mice. F, G The protein and mRNA expression levels of ER stress markers were significantly increased in the abdominal adipose tissue of mice in the HFD group. Blocking p-p65 reversed the above phenotypes in HFD-fed mice. H The protein expression levels of exosome markers were significantly increased in the abdominal adipose tissue of mice in the HFD group. Blocking p-p65 reversed the above phenotypes in HFD-fed mice. I, J The miRNA levels in the abdominal adipose tissue and serum of HFD-fed mice were significantly increased. Blocking p-p65 reversed the above phenotypes in HFD-fed mice. K‒M HFD-fed mice displayed elevated blood glucose levels, reduced glucose tolerance, and impaired insulin sensitivity. Blocking p-p65 reversed the above phenotypes in HFD-fed mice. *NCD and HFD comparison, #HFD and HFD+Bay11-7082 comparison, *P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.05, ##P < 0.01, and ###P < 0.001 indicate statistically significant differences.
