Fig. 6

NO66 promotes bone loss through activation of osteolytic factors secreted from PCa cells. a Gene expression levels of mouse genes in femoral bone injected with no cells, PC3-Vec (control) cells, and PC3-FlagNO66 cells. b Gene expression levels of mouse genes in primary murine osteoblasts co-cultured with PC3-Vec and PC3-NO66 cells. Representative data of three independent experiments is shown. Error bars indicate “mean ± SEM”; P < 0.05. c Western blot analysis for levels of OPG and RANKL in lysates of PC3-Nsh and PC3-sh