Fig. 7
Bioinformatics analysis for the genome wide distribution of the NO66 interactions. a Percentages of the interaction sites in the genome. b Representation via heat map for interaction sites after ChIPseq for NO66 and H3K9AC using respective antibodies. c An IGV snapshot of the peaks for NO66 and H3K9AC in target genes. d Quantitative RT-PCR for gene expression in PC3-Vec, PC3-FlagNO66, and PC3-NO66 (AKA) cells. NO66 (AKA) is a mutant for defective histone demethylase activity by substitution of catalytic histidine to alanine [20]. Error bars indicate “mean ± SEM”; P < 0.05. e ChIP analysis for the occupancy of NO66 in the chromatin of PC3-Vec and PC3-NO66 cells shows the NO66 interaction in the promoter region (Prox) and coding region (Int) of the genes DDIT3, MCL1, and CTNNB1
