Fig. 5: Effect of KBTBD11 expression on AKT ubiquitination and phosphorylation.
From: KBTBD11 loss promotes AKT hyperactivation and therapeutic vulnerability in prostate cancer
A 293 T cells were transiently transfected with the KBTBD11 and KBTBD11 truncation plasmids and harvested for Western blot analysis of KBTBD11-mediated ubiquitination of AKT. B, C 293 T cells were transiently transfected with the different His-Ub mutant plasmids and harvested for Western blot analysis of KBTBD11-mediated ubiquitination of AKT. D 293 T cells were transfected with the different plasmids for Myc-AKT truncation and mutation and harvested for Western blot analysis of KBTBD11-mediated ubiquitination of AKT. E, F 293 T cells were transiently transfected with the KBTBD11 and TRAF6 plasmids and harvested for Western blot analysis of KBTBD11-mediated ubiquitination of AKT. Representative images the His-Ub-K63O for TRAF6-mediated ubiquitination of AKT are shown in (E) and the His-Ub-K27O for KBTBD11-mediated ubiquitination of AKT are shown in (F). G DU145 cells were transiently transfected with the KBTBD11 and TRAF6 plasmids and harvested for Western blot analysis of the phosphorylation level of AKT.
