Fig. 3: Meflin involvement in tumor-initiating feature of OS cells.
From: Meflin is a druggable target using antibody-drug conjugates in progressive osteosarcoma
A Dunn cells were transfected with plasmids encoding Cas9 and either control guide RNA or that targeting the Meflin gene, followed by selection with puromycin to generate control or Meflin knockout (KO) cells (left panel). The cells were mixed with 0.36% soft agar and subjected to colony formation assay, followed by counting the number of tumorspheres under a microscope (middle and right panels). ***, P < 0.001. B 143B cells transduced with lentiviruses encoding EGFP or hMeflin were analyzed for Meflin expression by flow cytometry (left panel). 143B cells transduced with either EGFP or hMeflin were cultured in the same condition as in A, followed by counting the number of tumorspheres under a microscope (middle and right panels). ***, P < 0.001. C Control (CTL) and Meflin KO Dunn cells (left panel) or 143B cells transduced with EGFP or hMeflin (right panel) were plated on plastic dishes for indicated days, followed by counting viable cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays.
