Fig. 2: PMM2 enhances glycolytic activity to drive CRC progression. | Oncogene

Fig. 2: PMM2 enhances glycolytic activity to drive CRC progression.

From: PMM2 interacts with TRIM28 to recruit E2F4 and promote KIFC3-mediated tumor glycolysis and colorectal cancer progression

Fig. 2: PMM2 enhances glycolytic activity to drive CRC progression.

A Glucose uptake in HCT-116 cells with PMM2 knockdown (shPMM2-1). B ATP production in HCT-116 cells with PMM2 knockdown. C Lactate production in HCT-116 cells with PMM2 knockdown. D Extracellular acidification rate (ECAR) measured by Seahorse XFe analyzer in HCT-116 cells with PMM2 knockdown. E Oxygen consumption rate (OCR) in HCT-116 cells with PMM2 knockdown. F Western blot analysis of glycolytic enzymes (PKM2, LDHA) in HCT-116 cells with PMM2 knockdown. Effects of 2-deoxy-D-glucose (2-DG, 5 mM) on glucose uptake (G), ATP production (H), ECAR (I), and OCR (J) in PMM2-overexpressing HCT-116 cells. K Western blot analysis of PKM2 and LDHA in PMM2-overexpressing HCT-116 cells treated with 2-DG. CCK-8 (L) and colony formation (M) assays in PMM2-overexpressing HCT-116 cells with or without 2-DG. Data are mean ± SD (n = 3). **p < 0.01 vs. corresponding control.

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