Fig. 3: PMM2 interacts with TRIM28 via domain 1 to promote CRC progression. | Oncogene

Fig. 3: PMM2 interacts with TRIM28 via domain 1 to promote CRC progression.

From: PMM2 interacts with TRIM28 to recruit E2F4 and promote KIFC3-mediated tumor glycolysis and colorectal cancer progression

Fig. 3: PMM2 interacts with TRIM28 via domain 1 to promote CRC progression.

Co-immunoprecipitation (Co-IP) assays in HCT-116 and RKO cells showing interaction between endogenous PMM2 and TRIM28 (A: IP with anti-PMM2; B: IP with anti-TRIM28). C Co-IP of HA-tagged TRIM28 with FLAG-tagged full-length (FL) or domain-deleted PMM2 (Δ1, Δ2, Δ3) in HEK293T cells. Glucose uptake (D), Lactate production (E), and ECAR (F) in HCT-116 shCtrl or shPMM2 cells transfected with NC, PMM2-FL or PMM2-Δ1. G Western blot analysis of PKM2, LDHA, and PMM2 in HCT-116 cells grouped as in (D). CCK-8 (H) and wound healing (I) assays in HCT-116 cells grouped as in (D). Data are mean ± SD (n = 3). **p < 0.01 vs. corresponding control.

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