Fig. 4: PMM2 promotes TRIM28 nuclear translocation and KIFC3 transcription. | Oncogene

Fig. 4: PMM2 promotes TRIM28 nuclear translocation and KIFC3 transcription.

From: PMM2 interacts with TRIM28 to recruit E2F4 and promote KIFC3-mediated tumor glycolysis and colorectal cancer progression

Fig. 4: PMM2 promotes TRIM28 nuclear translocation and KIFC3 transcription.

A KIFC3 mRNA levels in CRC tissues vs. normal tissues (TCGA dataset). B Kaplan–Meier survival analysis of CRC patients with high vs. low KIFC3 expression (TCGA dataset). C Dual-luciferase reporter assay in HCT-116 cells transfected with wild-type (WT) or mutant (Mut1, Mut2, Mut3) KIFC3 promoter constructs, with or without TRIM28 overexpression. D Chromatin immunoprecipitation (ChIP) assay showing TRIM28 enrichment at the KIFC3 promoter (site 1, site 2, or site 3) in HCT-116 cells. E Western blot of TRIM28 in nuclear/cytoplasmic fractions of HCT-116 cells with PMM2 knockdown (Histone H3 and GAPDH as nuclear/cytoplasmic markers). F Immunofluorescence staining of TRIM28 in HCT-116 cells with PMM2 overexpression. Images of TRIM28 (green) and nuclei (blue) were merged. Scale bar, 20 μm. RT-qPCR (G) and Western blot (H) analysis of KIFC3 expression in HCT-116 cells with PMM2 knockdown. I ChIP assay showing PMM2 overexpression enhances TRIM28 binding to the KIFC3 promoter (site 1). J Dual-luciferase assay in HCT-116 cells co-transfected with PMM2 shRNA, TRIM28, and KIFC3 promoter constructs. Western blot analysis indicates TRIM28 and PMM2 expression. Data are mean ± SD (n = 3). **p < 0.01 vs. corresponding control. ns, not significant.

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