Fig. 7: CTPS1 mediates the malignant phenotypes of SNRPE in OC cells. | Oncogene

Fig. 7: CTPS1 mediates the malignant phenotypes of SNRPE in OC cells.

From: Spliceosomal component SNRPE drives cell proliferation by regulating CTP synthase 1 mRNA splicing in ovarian cancer

Fig. 7: CTPS1 mediates the malignant phenotypes of SNRPE in OC cells.

A, B The overexpression levels of SNRPE mRNA and protein (Flag-tagged) in HEY and SKOV3 cells were verified by qPCR and Western blot. C, D MTT and colony formation assays demonstrated that CTPS1 depletion significantly attenuated the enhanced proliferative and colony-forming capacities induced by SNRPE overexpression in HEY and SKOV3 cells. E, F Transwell assays demonstrated that CTPS1 knockdown markedly suppressed the enhanced migratory and invasive potential induced by SNRPE overexpression in HEY and SKOV3 cells. G Xenograft assays demonstrated that CTPS1 knockdown markedly weakened the enhanced tumorigenic capacity of OC cells induced by SNRPE overexpression in vivo. H, I Comparison of tumor volume and weight among the three groups shown in Fig. 7G. The P values were obtained by unpaired t-test (A) or one-way ANOVA (C, F, H, I). *P < 0.05, **P < 0.01.

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