Fig. 7: FBP1 K51 polyubiquitination regulates its downstream target genes, and the O-GlcNAcylation/polyubiquitination axis promotes tumour progression. | Oncogenesis

Fig. 7: FBP1 K51 polyubiquitination regulates its downstream target genes, and the O-GlcNAcylation/polyubiquitination axis promotes tumour progression.

From: O-GlcNAcylation of FBP1 promotes pancreatic cancer progression by facilitating its Lys48-linked polyubiquitination in hypoxic environments

Fig. 7

A RT‒qPCR results showing the expression levels of downstream target genes in AsPC-1 and SW1990 (FBP1-WT/K51R) cells with or without OGT knockdown. B Glucose uptake assays were performed in AsPC-1 and SW1990 (FBP1-WT/K51R) cells with or without OGT knockdown. C Lactate secretion assays were performed in AsPC-1 and SW1990 (FBP1-WT/K51R) cells with or without OGT knockdown. D MTT assays were performed to measure the growth of AsPC-1 and SW1990 (FBP1-WT/K51R) cells with or without OGT knockdown. E Colony formation assays were performed in AsPC-1 and SW1990 (FBP1-WT/K51R) cells with or without OGT knockdown, and the statistical results were calculated. F Xenograft experiments in nude mice were performed in AsPC-1 and SW1990 (FBP1-WT/K51R) cells with or without OGT knockdown, and the statistical results (i.e. tumour weight and volume) were analysed. G Schematic model of how the FBP1 O-GlcNAcylation/polyubiquitination axis functions in pancreatic cancer cells in a hypoxic environment.

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