Fig. 2: USP1 overexpression enhances the malignant behaviors of HCC. | Oncogenesis

Fig. 2: USP1 overexpression enhances the malignant behaviors of HCC.

From: Dual roles of USP1 in HELLS deubiquitination and SUMOylation drive EMT and FOLFOX-based chemoresistance

Fig. 2: USP1 overexpression enhances the malignant behaviors of HCC.

A Viability measured by CCK-8 in SK-Hep1 under ML323 treatment or shUSP1 knockdown. B Clonogenic proliferation assessed by colony-formation in SK-Hep1 under the indicated conditions. C Migration evaluated by Transwell in SK-Hep1 after ML323 or shUSP1. D Wound-healing migration in SK-Hep1 with shUSP1 and in Huh7 with USP1 overexpression. E Viability of Huh7 assessed by CCK-8 following USP1 overexpression. F Effects of USP1 overexpression on Huh7 colony formation. G Transwell migration of Huh7 with USP1 overexpression. H The association between USP1 profiles and EMT characteristics was evaluated using bioinformatics. EMT pathway scores were compared between high and low USP1 expression groups in the TCGA LIHC dataset. GSEA was performed to identify USP1-related pathways, and the correlation between USP1 and EMT-related markers in the TCGA dataset was analyzed. I Expression levels of EMT-related markers were analyzed by western blotting in HCC cells with USP1 overexpression or depletion. J Detection of EMT markers by immunofluorescence in HCC cells with different treatments. **P < 0.01; ***P < 0.001.

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