Fig. 1: The mitochondrial phospholipidome is unaltered in hepatocellular carcinoma (HCC).

A Images of representative liver dissected from 34-week-old mice (top) with a corresponding hematoxylin and eosin (H&E) stain (bottom). The inset H&E image is illustrating normal blood vessels (circular or oval areas that do not stain with H&E) in liver sections from LFD mice, or areas of HCC in WD + DEN/TAA treated mice, which also do not stain with H&E but are larger, irregularly shaped, and surrounded by nuclei (blue staining) (n = 6 mice per group). B Serum ALT activity was elevated in WD + DEN/TAA groups. The male and female LFD groups and female WD + DEN/TAA group had an n = 3. The male WD + DEN/TAA group had an n = 4. C Serum AST activity was elevated in WD + DEN/TAA groups (n = 3 per group). D Nuclei (Hoechst) and Ki-67 (proliferation marker, upregulated in HCC) fluorescent images of liver sections. Ki-67 staining was higher in WD + DEN/TAA treated liver sections (n = 6 per group). E Relative fluorescent intensity of Ki-67 staining from images shown in D (n = 6 per group). F PCA plot of mitochondrial lipids between non-tumor and tumor mitochondria (n = 6 per group). G Heatmap of select lipids that were differentially altered between non-tumor and tumor mitochondria (n = 6 per group). H The abundance of mitochondrial phospholipids was not different between non-tumor and tumor mitochondria (n = 6 per group). The data are presented as means ± S.D.