Fig. 4: Silencing PISD increases mitochondrial superoxide production, mitochondrial fission, and mitophagy. | Oncogenesis

Fig. 4: Silencing PISD increases mitochondrial superoxide production, mitochondrial fission, and mitophagy.

From: Targeting mitochondrial phosphatidylethanolamine alters mitochondrial metabolism and proliferation in hepatocellular carcinoma

Fig. 4

A Representative images of MitoSOX staining illustrating greater mitochondrial superoxide content in shPISD treated cells. B Quantification of MitoSOX fluorescent intensity from images presented in A (n = 30 per group). C Relative abundance (measured using metabolomics) of reduced glutathione (GSH) and glutathione disulfide (GSSG) in shSCR and shPISD treated cells (n = 3 per group). D The relative protein abundance of superoxide dismutase 2 (SOD2) was reduced in shPISD treated cells (n = 6 per group). E Western blot image of SOD2 protein abundance. F Western blot image showing protein abundances of markers of mitochondrial fission (DRP, MFF), fusion (OPA1, MFN1, MFN2), or mitophagy (Parkin) (n = 3 per group). G Densitometry quantification of images shown in F (n = 3 per group). H, I Representative fluorescent images of MitoTracker and LysoTracker colocalization. The white arrows are pointing to extracellular mitochondria stained green with MitoTracker. J Greater Manders colocalization coefficient of MitoTracker and LysoTracker indicates more mitophagy in shPISD treated cells (n = 5 per group). K Number of extracellular mitochondria puncta was greater around shPISD treated cells (n = 3 per group). The data are presented as means ± S.D.

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