Fig. 4: Functional impact of Rab37-mediated OPN secretion on lung cancer cell proliferation, migration, and invasion. | Oncogenesis

Fig. 4: Functional impact of Rab37-mediated OPN secretion on lung cancer cell proliferation, migration, and invasion.

From: Rab37-mediated OPN secretion enriches SPP1+ macrophages through autocrine–paracrine signaling to drive lung tumor progression

Fig. 4

A Schematic workflow of the CM transfer assay. Rab37 WT or KO THP-1 macrophages were stimulated with LCM for 24 h, followed by collection of CM with or without nOPN (5 µg/ml) or rOPN (100 ng/ml) supplementation, and subsequently applied to lung cancer cells. Cell proliferation assays of H460 (B) and H1299 (C) cells treated with the indicated CM, measured at different time points. D Transwell migration assay schematic illustrating lung cancer cells seeded in the upper chamber, with Rab37 WT or KO THP-1 macrophages placed in the lower chamber, in the presence or absence of nOPN (5 µg/ml) or rOPN (100 ng/ml) supplementation. Representative images (E) and quantification (F) of migration assays of H460 cells. Representative images (G) and quantification (H) of invasion assays of H1299 cells. Data are presented as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t–test.

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