Fig. 6 | Signal Transduction and Targeted Therapy

Fig. 6

From: ENO2 drives tumor cell-induced M2 macrophage polarization to promote colorectal cancer liver metastasis

Fig. 6The alternative text for this image may have been generated using AI.

ENO2 Induces M2 Macrophage Polarization to Drive Liver Metastasis. a Spatial feature plot showing the deconvoluted proportions of ENO2+ cancer cells and M2 macrophages in ST_liver4. The color scale indicates the predicted cell type weights per spot. Flow cytometry analysis of the macrophage markers CD86 and CD206 in co-culture systems (b) and xenograft tumors (c). d Representative flow cytometry plots showing the expression of CD206 and CD86 on macrophages co-cultured with patient-derived organoid (PDO) models. PDOs were transduced with ENO2 knockout sgRNA (Sg-ENO2 PDO) or ENO2 overexpression vector (ENO2 PDO) compared with their controls (Sg-NC PDO and Vector PDO). e, f Investigation of the effect of ENO2 expression on the formation of liver metastases in a splenic implantation model using C57BL/6 J mice. M2 polarization inhibitor (LPS), MIF inhibitor (ISO-1), M2 polarization agonist (IL-4), and MIF overexpression were used in the rescue experimental system

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