Fig. 6: SCFAs improving the transcriptional level of tight junction biomarkers in Caco-2 cells. | Translational Psychiatry

Fig. 6: SCFAs improving the transcriptional level of tight junction biomarkers in Caco-2 cells.

From: Alterations in short-chain fatty acid-associated gut microbiota and tight junction integrity in adolescent major depressive disorder

Fig. 6: SCFAs improving the transcriptional level of tight junction biomarkers in Caco-2 cells.

Reverse transcription-polymerase chain reaction (RT-PCR) analysis to investigate the expression of CLDN5 and tight junction protein 1 (TJP1) by propionate treatment (A) and butyrate treatment (B) for 24 h and 48 h. C The transcriptional expression of CLDN5 and TJP1 by lipopolysaccharide (LPS), LPS + P (propionate), and LPS + B (butyrate) treated for 24 h and 48 h. Different colors represent different concentrations of propionate and butyrate treatment. Error bars represent standard deviation (SD) from three independent experiments (n = 6). One-way ANOVA analysis followed by Dunnett’s multiple comparison test, with P-value ˂ 0.05 indicating statistically significant. ns, not significant; *, P-value < 0.05; **, P-value < 0.01; ***, P-value < 0.001.

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