Fig. 8: Interference with HDAC2 expression influences the cardioprotective effect of galangin 3-methyl ether (G3-ME) on PI3K-AKT signaling and cardiac hypertrophy. | Acta Pharmacologica Sinica

Fig. 8: Interference with HDAC2 expression influences the cardioprotective effect of galangin 3-methyl ether (G3-ME) on PI3K-AKT signaling and cardiac hypertrophy.

From: Galangin 3-methyl ether alleviates mouse hypertrophic cardiomyopathy via targeting HDAC2 and subsequently inactivating the PI3K-AKT signaling pathway

Fig. 8: Interference with HDAC2 expression influences the cardioprotective effect of galangin 3-methyl ether (G3-ME) on PI3K-AKT signaling and cardiac hypertrophy.

a The efficiency of HDAC2 knockdown (HDAC2-KD) in H9c2. b, c The mRNA expression levels of Nppa and Nppb in normal control (NC) or HDAC2-KD cells. n = 4 per group. d Representative images of NC or HDAC2-KD cell morphology were obtained through immunofluorescent staining of cTNT (red). e The cardiomyocyte size was analyzed from 8 confocal dishes, with all cardiomyocytes in the different groups being 95, 106, 101, 101, 102, and 95, respectively. f The protein expression levels of p-P85, P85, p-AKT and AKT in NC or HDAC2-KD cells. g The efficiency of HDAC2 overexpression (HDAC2-OE) in H9c2 cells. h, i The mRNA expression levels of Nppa and Nppb in control vector (Ctrl) or HDAC2-OE cells. n = 4 per group. j Representative images of Ctrl or HDAC2-OE cell morphology were obtained through immunofluorescent staining of cTNT (green). k The cardiomyocyte size was analyzed from 8 confocal dishes, with all cardiomyocytes in the different groups being 98, 84, 93, 81, 76, and 77, respectively. l The protein expression levels of p-P85, P85, p-AKT and AKT in Ctrl or HDAC2-OE cells. The values are mean ± SEM. *P < 0.05.

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