Fig. 7: Bicarbonate enhances TNF-α-induced RIP kinase activation in vivo. | Acta Pharmacologica Sinica

Fig. 7: Bicarbonate enhances TNF-α-induced RIP kinase activation in vivo.

From: Alkalization of the intracellular pH reprograms TNF-α signaling from inflammatory NF-κB activation to tumoricidal RIP kinase-dependent necroptosis in cancer cells

Fig. 7: Bicarbonate enhances TNF-α-induced RIP kinase activation in vivo.

Western blots showing the levels of pRIPK1, pRIPK3, pMLKL and total proteins in SK-HEP-1 (a) and HeLa (b) xenografts. Quantification of the ratios of the phosphorylated proteins to the total proteins. The data are presented as the means ± SDs (n ≥ 5 biological replicates). c, d Immunofluorescence staining of tumor tissues for pRIPK1 and pRIPK3. NF-κB (p65) nuclear translocation in SK-HEP-1 (e) and HeLa (f) tumors. Scale bar: 20 µm. The data are presented as the means ± SDs (n ≥ 5 biological replicates). g Representative electron micrographs showing mitochondria in SK-HEP-1 tumors. Unpaired t test. ns, P > 0.05; *P < 0.05; **P < 0.01; and ***P < 0.001.

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