Fig. 1 | Bone Research

Fig. 1

From: Deep imaging of LepR+ stromal cells in optically cleared murine bone hemisections

Fig. 1

Workflow of the optimized deep imaging protocol for optically cleared skeletal tissue. Steps for preparing the deep-imaging bone hemisections, including tissue fixation, decalcification, dehydration, embedding, and cryosectioning to generate bone hemisections; and immunostaining, tissue clearing, and mounting, with subsequent imaging. BABB, benzyl alcohol/benzyl benzoate (1:2, v/v) clearing solution

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