Fig. 2
From: Deep imaging of LepR+ stromal cells in optically cleared murine bone hemisections
Hematopoietic stem/progenitor cells locate near the vessels in adult bone marrow. a 2D projected images of thick specimen. Lineage−c-kit+ hematopoietic stem/progenitor cells are in close proximity to blood vessels, identified by laminin (Cyan) staining. A single HSPC is indicated by an arrow in the higher magnification images. b 2D projected images of 100-µm image stacks from a cleared bone marrow plug. α-catulin-GFP+c-kit+ hematopoietic stem cells (HSCs) are similarly situated near vessels, identified by laminin (Cyan) staining, with a single HSC highlighted by an arrow in the magnified views. Quantitative analysis of the distance between HSCs and randomly selected spots to the nearest blood vessel (c) and endosteum (d). Data are representative of 7 experiments with one mouse per experiment. e 2D projected images of 50-µm image from a cleared bone hemisection. Blood vessels, identified by endomucin labeling, and LepR+ cells in young (2-month-old) and aged (20-month-old) wild-type mice, visualized after clearing with BABB. All images are representative of 3 experiments with one mouse per experiment per age
