Fig. 4

Characterization of bone marrow vasculature in long bones. In 100-µm-thick bone sections from 2-month-old Oln^mTomato/+; Col1a1*2.3-eGFP mice, Col1a1*2.3-eGFP⁺ osteoblasts, which also express Oln-mTomato, were identified on both trabecular (a) and cortical (b) bone regions. c The transcortical blood vasculature within the cortical bone is visualized by endomucin staining (marked by white asterisk) in 100-µm-thick bone sections from the same mouse model. d 2D projected images of 200-µm image stack from a cleared bone hemisection. Transcortical vessels, labeled with endomucin (red), are evident in the cortical bone (blue, SHG) of the tibia from a wild-type mouse, following clearing with the BABB method. All images are representative of 3 experiments with one mouse per experiment