Fig. 6

Characterization of a novel monoclonal antibody against LepR. a, b Flow cytometric analysis of enzymatically dissociated bone marrow cells from 2-month-old wild-type or Lepr^cre/+; Rosa26^tdTomato mice demonstrates the identification of a putative LepR⁺ cell population using a novel anti-LepR antibody. The absence of this population in the FMO (Fluorescence Minus One) control confirms antibody specificity. b Anti-LepR staining overlapped with LepR⁺ cells identified by Lepr^cre/+; Rosa26^tdTomato mice. Data are representative of 3 experiments with one mouse per experiment. c Immunofluorescence staining of a 50-µm-thick femur bone marrow section from a Lepr^cre/+; Rosa26^tdTomato mouse with anti-LepR antibody. d Immunofluorescence staining of a 50-µm-thick bone marrow section using anti-LepR and anti-endomucin antibodies. Images are representative of 3 experiments with one mouse per experiment. e Application of the anti-LepR antibody in human samples was demonstrated through staining of enzymatically dissociated human bone marrow cells. Data are representative of 3 independent experiments with 3 human specimens. f Multilineage differentiation potential of CFU-F colonies formed by human LepR⁺ cells in panel (e). All Images are representative of 3 independent experiments with 3 human specimens