Fig. 8

Characterization of a Lepr^mTagBFP2 reporter allele. a Flow cytometric analysis of enzymatically dissociated femur bone marrow cells from 2-month-old wild-type and Lepr^mTagBFP2/+ mice, demonstrating that most Lepr-mTagBFP2⁺ stromal cells were LepR⁺. 3 mice from 3 independent experiments. Deep imaging of femur bone hemisections from 2-month-old Lepr^mTagBFP2/+ mice shows that Lepr-mTagBFP2⁺ cells were perivascular, locating on endomucin⁺ vessels (b), and Lepr-mTagBFP2⁺ cells were all LepR⁺ (c). d Lepr-mTagBFP2⁺ cells are observed in the hypothalamus of Lepr^mTagBFP2/+ mice. Data and images are representative of 3 experiments with one mouse per experiment