Table 2 Antibodies successfully used with this method
From: Deep imaging of LepR+ stromal cells in optically cleared murine bone hemisections
Antibody | Recommended working concentration | Company | Cat. no. |
|---|---|---|---|
Primary antibodies | |||
Chicken anti-green fluorescent protein | 40 μg/mL | Aves Labs | GFP-1020 |
Rabbit anti-laminin Ab-1 | 1:200 | Thermo Fisher Scientific | RB-082-A0 |
Goat anti‑discosoma tdTomato | 12 ng/μL | LifeSpan Biosciences | LS-C340696 |
Rat anti-endomucin (V.7C7) | 1:100 | Santa Cruz | sc-65495 |
Goat anti-endomucin | 1 ng/μL | R&D Systems | AF4666 |
Goat anti-VEGFR3/Flt-4 | 1:100 | R&D Systems | AF743 |
Goat anti-VE-Cadherin | 1:100 | R&D Systems | AF1002 |
Rabbit anti-DsRed | 1:250 | Takara Bio | 632496 |
Rabbit anti-laminin 1 + 2 | 2 ng/μL | Abcam | ab7463 |
Rabbit anti-peripherin | 1:250 | Abcam | ab4666 |
Biotin goat anti-CD117/c-Kit | 1:250 | R&D Systems | BAF1356 |
Rat anti-CD31/PECAM-1 (MEC 13.3) | 1:100 | Santa Cruz | sc-18916 |
Rabbit anti-Leptin Receptor | 1:100 | Abcam | ab318272 |
Biotin goat anti-Leptin Receptor | 2 ng/μL | R&D Systems | BAF497 |
Biotin hamster anti-CD3e | 1:100 | BD Biosciences | 51-01082 J |
Biotin rat anti-CD11b | 1:100 | BD Biosciences | 51-01712 J |
Biotin rat anti-CD45R | 1:100 | BD Biosciences | 51-01122 J |
Biotin rat anti-Ly-6G and Ly-6C | 1:100 | BD Biosciences | 51-01212 J |
Biotin rat anti-Ter119/Erythroid cells | 1:100 | BD Biosciences | 51-09082 J |
FITC mouse anti-Actin, α-smooth muscle | 1:250 | Sigma-Aldrich | F3777 |
APC rat anti-Ly-6A/E (Sca-1) | 2.5 ng/μL | Biolegend | 122518 |
Rabbit anti-tRFP antibody | 12 ng/μL | Evrogen | AB233 |
Secondary antibodies | |||
Donkey anti-goat IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 555 | 4 ng/μL | Thermo Fisher Scientific | A21432 |
Alexa Fluor 488 AffiniPure F(ab’)2 fragment, donkey anti-rabbit IgG (H + L) | 1.2 ng/μL | Jackson ImmunoResearch | 711-546-152 |
Alexa Fluor 647 AffiniPure F(ab’)2 fragment, donkey anti-rabbit IgG (H + L) | 1.2 ng/μL | Jackson ImmunoResearch | 711-606-152 |
Alexa Fluor 488 AffiniPure F(ab’)2 fragment, donkey anti-chicken IgY (IgG) (H + L) | 1.2 ng/μL | Jackson ImmunoResearch | 703-546-155 |
Alexa Fluor 647 AffiniPure donkey anti-goat IgG (H + L) | 4 ng/μL | Jackson ImmunoResearch | 705-605-147 |
Donkey anti-rabbit IgG (H + L) highly cross-adsorbed secondary antibody, Alexa Fluor 546 | 1:250 | Thermo Fisher Scientific | A10040 |
APC Streptavidin | 1:200 | Biolegend | 405207 |
Alexa Fluor 594 AffiniPure Fab fragment donkey anti-rat IgG (H + L) | 1:500 | Jackson ImmunoResearch | 712-587-003 |
