Fig. 2

μCT and histomorphometric analysis showed OB-HFD mice have trabecular and cortical bone loss due to an increase in osteoclasts. a Tibial trabecular bone volume per total volume (Tb. BV/TV, %; n = 6-16). b Trabecular spacing (Tb.Sp, mm). c Trabecular number (Tb.N, mm^-1). d Trabecular thickness (Tb.Th, mm). e Tibial cortical area per total area (Ct.Ar/Tt.Ar, %). f Representative coronal, mid-shaft μCT images of the proximal tibia (scale bar = 1 mm). g Tibial cortical thickness (Ct.Th, mm). h Cortical polar moment of inertia (Ct. pMOI, mm4). i Representative femoral images with immunohistochemistry for TRAP (pink) and F4/80 (brown) (scale bar = 20 μm). j Number of osteoclasts per bone perimeter (N.Oc/BPm, number per mm; n = 12-14). k Number of osteoblasts per bone perimeter (N.Ob/BPm, number per mm). l Mineralizing surface per bone surface (MS/BS, %). m Mineralizing apposition rate (MAR, μm/d). n Bone formation rate per bone surface (BFR/BS, %). Analyses for a and d–h were performed as a One-way ANOVA with Tukey’s multiple comparisons test. Analyses for b, c were performed as a Kruskal-Wallis test with Dunn’s multiple comparison