Fig. 3

BMAT expansion in OB-HFD mice did not have an inflammatory phenotype and increased osteoclast differentiation. a Representative H&E images of the BMAT within the proximal tibia. b Number of BM adipocytes within the proximal tibia (BM.Ads/Ma.AR, number/mm²). c Average size of the BMAT within the proximal tibia (μm²). d Representative TRAP⁺ images after 5 days of osteoclast differentiation when co-cultured with BMAT. e Number of TRAP⁺ cells after 5 days of osteoclast differentiation when co-cultured with BMAT (n = 6; each dot represents an averaged technical replicate). f Average diameter of TRAP⁺ cells after 5 days of osteoclast differentiation when co-cultured with BMAT. g Immune and inflammatory gene expression of BM adipocytes relative to TBP (n = 7; represented as the mean with individual data points). h Immune and inflammatory gene expression of visceral, perigonadal WAT (gWAT) relative to TBP (n = 10; represented as the mean with individual data points). Analyses for b, c were performed as a One-way ANOVA with Tukey’s multiple comparisons test. Analyses for e, f were performed as a 2-way ANOVA with Tukey’s multiple comparisons test. Analyses for g, h were performed as a 2-way ANOVA with Šídák’s multiple comparisons test