Fig. 6

BMAT depletion in obese mice improved BM immunosuppression through a decrease in PD-L1⁺ myeloid cells. a Number of BM adipocytes within the proximal tibia (BM.Ads/Ma.AR, number/mm²; n = 10-13). b Average size of the BMAT within the proximal tibia (μm²). c Representative H&E images of the BMAT within the proximal tibia (scale bar = 250 μm). d Immune and inflammatory gene expression of BM adipocytes from LFD mice relative to TBP (n = 8; represented as the mean with individual data points). e Immune and inflammatory gene expression of BM adipocytes from OB-HFD mice relative to TBP (n = 8; represented as the mean with individual data points). f F4/80 IHC of gWAT (red arrows indicate minimal macrophage infiltration in LFD-fed mice). g Number of PD-L1⁺ myeloid cells. h Number of PD-1⁺ osteoclast precursor. i Serum procollagen type I N-propeptide (P1NP) levels (n = 8). j Serum C-terminal telopeptide of type 1 collagen (CTX-1), a bone resorption marker. k Serum TRAcP 5b levels. Analyses for a, b and g–k were performed as 2-way ANOVA with uncorrected Fisher’s LSD. Analyses for d, e were performed as multiple unpaired t-tests and multiple Mann-Whitney tests, respectively