Fig. 2: SYK inhibitor decreases clonogenic and invasive potential of GI-NETs by increasing E-Cadherin levels. | Cancer Gene Therapy

Fig. 2: SYK inhibitor decreases clonogenic and invasive potential of GI-NETs by increasing E-Cadherin levels.

From: Spleen tyrosine kinase (SYK) inhibition suppresses growth of gastrointestinal neuroendocrine tumor cells: a pilot study in two cell lines

Fig. 2

For the invasion assay of COLO320DM (A) and GOT1 (B), cells were seeded on the Matrigel-coated upper chamber of the transwell. Twenty-four hours later, migrated cells were fixed, stained, and counted (n = 3 independent experiments, minimum 2 technical replicates). qRT-PCR of E-cadherin expression levels in COLO320DM (C) and GOT1 (D) cells following treatment with SYK inhibitor. Significance was calculated using one-way ANOVA. Data are expressed as mean ± SEM; **P < 0.01, ****P < 0.0001 vs. DMSO, significance was calculated by Anova using Dunnett’s multiple comparison test. Changes in colony number and dimension of COLO320DM (E) after treatment with SYK inhibitor, with representative images of colony formation assays. Colonies were quantified using Image J Software and results show the percentage of colonies formed after treatment with the indicated concentrations of the drug (surviving fraction), corrected according to the plating efficiencies of the corresponding controls. Data are shown as mean ± SEM of at least three independent experiments, *P < 0.05 vs vehicle cells significance was calculated by two-way ANOVA using Šidák’s multiple comparison test.

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