Fig. 5

Enhanced differentiation correlates with mTOR-signaling. a Western blot analysis of Egfr^wt and Egfr^−/− osteoblasts under differentiation conditions; isolated on differentiation day 14. b Western blot analyses of Egfr^wt and Egfr^−/− osteoblasts under differentiation conditions on days 6, 9, 12, and 15. c Immunohistochemical staining of p-mTOR, p-4E-BP1 and p-S6 on trabecular bone sections from distal femurs of P7 Egfr^wt and Egfr^−/− littermates; scales: 200 µm (lower magnification) and 20 µm (higher magnification). d Quantification of IHC staining, shown as positive cells per bone perimeter (B.Pm); n ≥ 3